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The reported sulfatase-based method is a robust tool for the discovery of unknown microbiota-derived metabolites in human samples. Rapid diagnostic testing (RDT) has been shown to be associated with improved clinical outcomes. To evaluate the clinical outcomes of using RDT paired with well-defined pharmacist-directed antimicrobial stewardship programme (ASP) guidance to achieve targeted treatment in patients with bacteraemia. In this quasi-study, a retrospective (pre-intervention) phase was compared with a prospective (post-intervention