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About 12.2% of retests were done within 3months and 29% had≥2 retests within 1year. 57% of retests were in those initially vitamin D replete (50nmol/L). The annual cost of inappropriate testing was €61,976. One in four patients were retested and this varied by age, gender and patient location. Over 10% of retests were inappropriately early (3months), a third too frequent and over half were in replete individuals incurring significant costs. Clear guidance for GPs on minimum retesting intervals is needed, as well as laboratory orderi