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Fructokinase (FRK) mediates fructose phosphorylation to regulate the carbon flow and its assignment to sink tissues. Out of five HbFRKs in the genome of the rubber tree, three (HbFRK1-3) that were highly expressed in latex (cytoplasm of laticifers) were isolated and examined. According to phylogenetic analysis and intracellular location experiment, both HbFRK2 and HbFRK3 were highly possible to be expressed in cytosol, while HbFRK1 was in plastid. As the predominant isoform in laticifers, HbFRK2 had the highest transcripts, followed by H