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The seedlings improved the most under the combined treatment of BC20N150, wherein the seedling heights, photosynthesis and total chlorophyll improved by 22 %, 70 % and 40 %, respectively, compared to those treated solely with BC20. Further increase of nitrogen retention in the soil by the BC40 did not further improve the growth of the seedlings, suggesting the possible mechanisms involve nutrient uptake and usage dynamic in the seedlings. The BC amendment alleviated the antagonist effect from simulated nitrogen deposition that suppresse