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This study aimed to find a new source of anti-leukemia agents from Vietnamese medicinal plants. The human leukemia cell lines TCCY, KU-812, TCC-S, KOPB-26, and HL60 were used. The crude ethanol extracts of 17 medicinal plants were collected and evaluated for their cytotoxicity against these leukemia cell lines by the trypan blue dye exclusion test. Morphological changes of cells were observed under phase-contrast inverted microscope. Bioactive compounds were evaluated using the method described by Ciulei. 2,2-diphenyl-1-picrylhydrazyl (D