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We identified a potent and selective small molecule inhibitor of HDAC3 with direct antimicrobial activity against Mtb and modulation of macrophage signaling pathways. We identified a potent and selective small molecule inhibitor of HDAC3 with direct antimicrobial activity against Mtb and modulation of macrophage signaling pathways.Glucosinolates, an important class of secondary metabolites in cruciferous vegetables, play a crucial role in protecting plants from stress-related damage. The mechanism of glucosinolate synthesis under short-