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With increased surface roughness Ra of the specimens, cell spreading was reduced. Cell viability after 24h correlated linearly with the tetragonal phase ratio of the specimens. Gene expression after 24h and 3 d was comparable on all specimens irrespective their surface roughness or monoclinic to tetragonal phase ratio. Significance Smooth zirconia surfaces with a high tetragonal phase ratio revealed best surface conditions for MG-63 osteoblastic cells and may be considered to design the endosseous part of zirconia implants.Background CD3
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