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Organisms were confronted with various levels of rutile (0, 5, 50, 100 μg/L) at control (18 ± 1.0 °C) and enhanced (22 ± 1.0 °C) conditions. Histopathological and biochemical changes were examined in mussels after 28 times of visibility. Histopathological evaluation revealed similar changes on mussels' gills and digestive glands with increasing rutile levels at both temperatures. Biochemical markers indicated that