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Superfine grinding (SG) technology has attracted considerable interest in meals and medication researcher industries. Polysaccharides in superfine powder of Gynostemma pentaphyllum Makino (GPP) had been extracted utilizing three techniques, including heated water extraction (HWE), ultrasound-assisted hot extraction (UAE), and microwave-assisted hot removal (MAE), while the purified polysaccharides were particularly denoted as GPWP, GPUP, and GPMP, respectively. The possible frameworks of polysaccharides were inv