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Chiral metal-organic frameworks (CMOFs) have been explored as potential chiral stationary phases (CSPs) for chiral high performance liquid chromatography (HPLC). However, their application is still hindered by the low column efficiency, high back pressure and the difficulty in column packing due to the irregular shapes and wide size distributions of CMOF particles. Here we report an efficient one-pot method for the immobilization of chiral MOF [Cu2((+)-Cam)2Dabco] (Cu2C2D) onto microspheric silica particles, generating a uniform core-s