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In order to produce 2,4-diacetylphloroglucinol (2,4-DAPG) in E. coli, the key synthases coding by phlACBD gene cluster from the strain Pseudomonas fluorescens CHA0 were overexpressed in E. coli BL21 (DE3). The marA, phlE and acc genes were also overexpressed to enhance 2,4-DAPG biosynthesis. Then the fermentation conditions were optimized to improve the concentration of 2,4-DAPG. The results showed that the recombinant E. coli could produce few 2,4-DAPG with only the phlACBD gene cluster. The synthetic ability of 2,4-DAPG could be increa
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