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Non-metric multidimensional scaling and PICRUSt2 functional analysis revealed differential abundance for the bacterial β-lactam-related functions when contrasting the genotypes of F. oxysporum. These results indicated that the Fo18438 β-lactamase domain provides F. oxysporum with the advantage of growing into the soybean rhizosphere, where β-lactam antibiosis is involved in microbial competition. Accordingly, this study highlights the capability of an F. oxysporum gene for altering the soybean rhizosphere and taproot microbiota.Communica