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In this study, we performed transcriptome analysis using two diverse hereditary cucumber cultivars, L-9 and A-16 grown under normal as well as heat stress. L-9 exhibited heat-tolerance phenotype with greater superoxide dismutase chemical (SOD) chemical activity and reduced malondialdehyde (MDA) content than A-16 under heat stress. RNA-sequencing unveiled that a total of 963 and 2778 genes are differentially expressed between L-9 and A-16 under normal as well