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In this study, Ocimum basilicum (a proven broad spectrum medicinal plant for broad-spectrum pharmacological activities) leaf extract was used as conjugates for the fabrication of silver nanoparticles (AgNP). Color change of the reaction mixture and UV-Visible spectrophotometry indicated the fabrication of silver nanoparticles, further X-ray diffraction (XRD) crystallography, scanning electron microscopy (SEM), transmission electron microscopic images (TEM), and Selected area electron diffraction (SAED) confirms the purity, monodispersity