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Hence, new methods that take into account ghost introgression will need to be developed. © 2020 The Authors. BioEssays published by WILEY Periodicals, Inc.BACKGROUND This study examined the prevalence of free-living Acanthamoeba in domestic tap water in the greater Sydney region, Australia, and determined any seasonal variation in prevalence. METHODS Fifty-four participants were included in this study following approval from an institutional human research ethics committee. Each participant self-collected two samples (one in summer and a
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