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The lower expression level of ALKb, compared with ALKa, resulted in depleted intermediate chains and increased short chains of amylopectin, thus affected the thermal and pasting properties of NILs' grains. Also, the data revealed both low-GT alleles were mainly found in temperate japonica, but more ALKb was found in other subpopulations such as indica as compared to ALKa. Conclusions Overall, all the results suggested that the function between two low-GT alleles was different, and the distribution of ALKb was much wider than that of ALK
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