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The trace amount of antibiotics in water can be enriched in the human body through the food chain, leading to extremely harmful effects on people's health. Therefore, it is urgent to develop new methods to detect trace pollutants in various aquatic phase. An analytical method utilizing the synergistic effect between the sensing strategy and catalytic material with high electron transfer capacity can be used to detect trace antibiotics. In this paper, an ultrasensitive impedimetric aptasensor was fabricated by the synergy between function