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The obtained M3 solitary mutants of Bn.MRP5.A10 and Bn.MRP5.C09 were combined by crossing to create double mutants. Simultaneously, crosses had been done utilizing the non-mutagenized EMS donor genotype to cut back the backdrop mutation load. Double mutants identified from the F2 progeny of direct M3 crosses and BC1 flowers revealed 15% lowering of PA articles with no considerable variations in Pi. We have been discussing the function of BnMRP5 paralogs and the benefits for reproduction Bnmrp5 mutants i