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The major change was noticed in E3 protein hydropathacity -0.281 to -0.362, in E2 isoelectric point (pI) 8.24 to 8.37, Instability index 66.08 to 71.062, aliphatic index varies from 74.69 to 68.59 and E3 75.79 to 70.05. In nsP1 protein pI varies 6.62 to 8.04, while no other change was observed in structural and nonstructural protein. The linear B- cell epitopes, position, and number were found to be varied with the mutation. The molecular characterizations of WGS demonstrate the observation of proteins stability, antigenicity with resp