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We additionally revealed that 1.5-μM DAP had no effects on Cav1.2 or Cav2.1 present. Next, we utilized voltage imaging to show that 1.5- or 100-μM 3,4-DAP broadened the AP waveform in a dose-dependent manner, separate of Cav1 calcium stations. Eventually, we demonstrated that 1.5- or 100-μM 3,4-DAP enhanced transmitter release in a dose-dependent way and also this result was also separate of Cav1 channels. From the results, we conclude that re