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good. Instruments able to capture specific needs of people with LSS in terms of activities and participation are lacking. Instruments able to capture specific needs of people with LSS in terms of activities and participation are lacking. Raman spectroscopy (RS) applied to surgical guidance is attracting attention among scientists in biomedical optics. Offering a computational platform for studying depth-resolved RS and probing molecular specificity of different tissue layers is of crucial importance to increase the precision of these t