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Polymerase chain reaction detected that these isolates harboured one or more β-lactamase genes (bla , bla , bla or bla ). TEM-1, TEM-34, CTX-M15, CTX-M9 and CTX-M61 type ESBLs were identified through sequencing. The novel Salmonella cefotaxime-hydrolysing β-lactamase, CTX-M61/TEM-34, detected in this study showed the emergence of new CTX-M-type ESBLs in Tunisia. There were found 33 different multidrug resistance (MDR) patterns. These findings highlighted the proliferation of ESBLs and MDR in Salmonella Kentucky and Typhimurium isolates