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Using chemical fractionation of CAN296, we identified the CBD small fraction once the primary inducer for the anti-tumoral activity. We succeeded in defining the mixture of CBD with cannabichromene (CBC) or tetrahydrocannabinol (THC) present in minute concentrations into the herb, producing a synergic impact that mimics the extract's complete effect. The cytotoxic impact might be maximized by combining CBD with either CBC or THC in a ratio of 21. This research reveals using decarboxylate