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001), peaking at 2,444. After the intervention, prevalence decreased by 59 per day (95% CI, -72 to -46; P .001). Epidemiological investigations determined that the most common source of HCW infection (58%) was a coworker. Universal masking in general hospitals reduced the risk of hospital-acquired COVID-19 among HCWs. Universal masking combined with uniform definitions of HCW exposure and criteria for quarantine limited the absence of HCWs from the workforce. Universal masking in general hospitals reduced the risk of hospital-acquir