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Right here, we report the generation of a series of homozygous HEK 293T cellular outlines with different variety of CAG repeats at the HTT locus and demonstrate their effectiveness for testing therapeutic reagents. In addition, using the CRISPR-Cas9 system, we corrected the mutation in HD human caused pluripotent stem cells and created a knock-out regarding the HTT gene, thus offering a thorough pair of isogenic cell outlines for HD investigation.When euryhaline fish move between fre