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The kinetic constants through the two methods were cross-validated and discovered to be in contract at the single-cell degree. The variation of observed price constant involving the two techniques is all about 0.009 s-1, that is comparable level whilst the cell-to-cell variants. This outcome verifies that both techniques can help measure whole-cell binding kinetics, and the integration gets better the reliability and convenience of the measurement.G-quadruplexes are non-canonical four st