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0 (IBM Corp., Armonk, NY). A p-value of less than or equal to 0.05 was considered significant. Main results A total of 1308 (52.3%) neonates were males, while 1193 (47.7%) were females. Serum TSH levels were ranged from 0.01-73.9 µU/ml. The mean ± SD was (7.60 ± 6.02 µU/ml); the cutoff point was 25 µU/ml. Conclusion The prevalence of congenital hypothyroidism among Saudi neonates in Bisha Province is 1834, which is the highest in the rejoin. The prevalence of hypothyroidism from the total screened population was (0.12%). We recommended sc