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Authentication of geographical origin is essential to the food safety of refined sugar. This study aimed to determine the geographical origin traceability and authenticity of refined sugar in China. Ultra performance liquid chromatography-quadrupole time-of-flight Mass Spectrometry (UPLC-QTof-MS), instead of conventional stable isotope ratio mass spectrometer (IRMS), was used to detect the mass fragment ratios (Rδ-sucrose and Rδ-glucose) of refined sugar. These ratios could reflect the cultivation practice and environmental conditions.