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In contrast, a significant avoidance response was observed in the 25 and 50 t ha-1 treatment and according to ISO guideline 17512 there is a negative impact of the Miscanthus ash on soil habitat function at 25 t ha-1 and above as more than 80% of earthworms were in the control soil. It is suggested that this negative effect on soil habitat function could be attributed to a range of factors including the presence of heavy metals in the ash and a change in substrate pH, texture and/or osmotic stress. Further laboratory-based studies conducted over ext
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