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The effective fabrication associated with biosensor had been characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM). The DNA biosensor performances had been evaluated utilizing a synthetic SARS-CoV-2 genome and 20 clinical RNA samples from healthy and infected individuals through EIS. The evolved DNA biosensor can detect as low as 1 content per μL for the N gene within five minutes with a LOD of 0.50 μM. Interest