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•The parameters of the method are adjusted using both observers opinion and the well-known reference image quality assessment BRISQUE (Blind/Referenceless images spatial quality Evaluator) score. © 2020 The Authors.Hydrogen peroxide (H2O2) is an important signal molecule produced in animal and plant cells. The balance of H2O2 between the intra- and extracellular space is regulated by integral membrane proteins, which thereby modulate signaling. Several methods have been established to analyze aquaporin mediated transport of H2O2 in wh