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The important thing aspects including the particle size and amount of packing material, in addition to kind and number of elution solvent, which impact PT-SPE device performance were optimized. A packing material of 40 mg/0.20 mL in a ratio of 4/6 (MIPs/HDPE) and treatment temperature of 150 °C had been chosen. By the determination with high-performance liquid chromatography (HPLC-SPD), the removal product ended up being found to have a great removal recovery for a 2,4-D pond liqu