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Long and real time imaging of cell membrane is very important for better understanding of cell performances in physiological and pathological processes. Nowadays, fluorescence probe analysis has become an indispensable tool for monitoring cell membrane. Herein, a novel fluorescent probe based on specific recognition of GABAA receptor was developed for imaging cell membrane. The probe synthesized in this work has been successfully applied to image different kinds of cell membrane with some advantages over the reported probes. Moreover,