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The statistical approaches (Plakett-Burman and response area methodology) were more employed for the optimization of medium elements. Moderate structure 0.1% w/v sugar, 0.3% w/v yeast plant, 0.03% w/v magnesium sulphate, 0.20% w/v potassium dihydrogen phosphate and 2.5% w/v salt chloride with inoculum size (1% v/v) ended up being suitable for greater GGT titres (449 U ml-1). Time kinetics showed the stability of enzyme as much as 96 h of incubation suggesting its application within the pr
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