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The stability study of the biosurfactant reveals that the emulsifying activity was much stable at various ranges of temperature (4-120 °C), pH (2-12), and NaCl concentration (2-10%, w/v) even on the 7th day. The purified biosurfactant PU1 was found to be effective in oil recovery and showed 68.53 ± 3.07% of oil recovery in the sand pack column under saline condition, which was higher than anionic surfactant sodium dodecyl sulphate (SDS). The physico-chemical properties and the stability studies of the biosurfactant suggested that it has