https://mln0128inhibitor.com/e....valuation-regarding-
We immunized rabbits with recombinant human B7-H3 (CD276) protein, made a phage-displayed rabbit VH solitary domain library with a diversity of 7 × 109, and isolated two binders (A1 and B1; also called RFA1 and RFB1) from phage panning. Both rabbit VH single domains exhibited antigen-dependent binding to B7-H3-positive tumor mobile outlines not B7-H3 knockout tumefaction cell outlines. Our research reveals that protein immunization followed closely by phage display evaluating