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In this study, 10 Aeromonas and seven Pseudomonas bacterial strains had been separated from spoiled grass carp and identified. Twelve of seventeen bacterial strains revealed high-potential of biofilm development and 14 of 17 can create extracellular protease. So that you can explore the spoilage capability of dual-species, the sterile grass carp fillets had been inoculated with mono- and dual-species of Aeromonas salmonicida and Pseudomonas azotoformans strains. The results disclos