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The tp0548 gene, hypothesized to encode for an outer membrane protein(OMP), was originally used in the ECDCT for molecular typing of T. pallidum. It plays an important role in the molecular epidemiology of Treponema, because it is not only an important locus of multiple typing approaches, but also suitable for strain-typing of multiple Treponema subspecies. A 27-year-old Chinese man attended the Institute of Dermatology, Chinese Academy of Medical Sciences Sexually Transmitted Disease (STD) Clinic in Nanjing, China due to a genital ulce