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Hearing loss (HL), with more than 100 gene loci, is the most common sensorineural defects in humans. The mutations in two and (Gap Junction Protein Beta 2, 6) genes are responsible for nearly 50% of autosomal recessive nonsyndromic hearing loss. The aim of the present study was to evaluate polymorphisms of 111CT (rs7329857) and 337GT (rs7333214) in (encoding connexin 26) and (encoding connexin 32) genes, respectively. In this study, 32 blood samples were obtained from Iranian patients with HL defect and 32 normal blood samples wer