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Here, we describe a macroscopic DNA film that has been produced enzymatically. This DNA film was later coated with thrombin (T utilizing an aptamer-protein conversation, to expedite hemostasis over a sizable location. The DNA film coated with TB (DNA patch) dramatically enhanced plasma hemostasis by acting as a coagulative scaffold for TB, in addition to holding localized TB. This research elucidates the advantages of making use of enzymatic amplification-based DNA structures into