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In this study, the secondary metabolites of a lichen-derived actinomycete strain Amycolatopsis sp. YIM 130687 were investigated intensively by using three different media (4#, 302#, and 312#) for fermentation. A total of 21 compounds were isolated from the fermented extraction of the strain. The structures of all compounds were identified by the examination of HRESIMS and NMR spectra. Compounds 1-3, 5, 6, 21 were only found in the cultivation on 302# medium, while compounds 4, 9-11 were only obtained when the strain was cultured on 312# medium. On the other