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Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#cryptofish 1-5) and one grouped separately (#cryptofish 7). In the present study, after phylogenetic analyses of #cryptofish1, #cryptofish2, #cryptofish4, #cryptofish5 and #cryptofish7 at the actin locus, the presence of two major clades was confirmed.