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Moreover, gene expression patterns can provide crucial complementary information to improve risk assessment, and monitoring of affected systems. The data reported in this article was used to depict the effects of single or combined exposure to norfluoxetine and venlafaxine and identify biomarkers of exposure to these compounds of interest to diagnose exposure and routine monitoring.This data article includes information on the impact of gold mining along five zones of a tropical river in the Pacific region of Colombia. The