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In the studied levels (40 mg·L-1, 80 mg·L-1, 120 mg·L-1), ZnO NPs had obvious impacts regarding the task of Proteobacteria. Adverse effects were specifically evident in sulfur and nitrogen biking bacteria such as for instance Sulfitobacter, unidentified_Nitrospiraceae, Thauera, and Azoarcus. The alpha variety index of microbial community failed to reflect more powerful biological toxicity into the teams with high NP levels (80 mg·L-1, 120 mg·L-1) than the group with low NP focus (40 mg·L-1). The typical well color development (AW