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Additionally, this review summarizes recommendations and outcomes of the expert meeting held by the BuRO in 2018, in order to contribute to the hazard identification and risk assessment process of ingested TiO2.The use of allium extract containing propyl propane thiosulfonate (PTSO) as hen feed supplement was evaluated to demonstrate its positive effect on egg production and intestinal microbiota modulation. The study was carried out on 90 laying hens whose feed was supplemented with allium extract for 28 days. Nutritional properties of