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This study modified an electromyography-assisted optimization approach for predicting lumbar spine loading while walking with backpack loads. The modified-electromyography-assisted optimization approach eliminated the electromyography measurement at maximal voluntary contraction and adopted a linear electromyography-force relationship. Moreover, an optimal lower boundary condition for muscle gain was introduced to constrain the trunk muscle co-activation. Anthropometric information of 10 healthy young men as well as their kinematic, kinetic