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Chicks were individually weighed weekly (gm); also, the weight gain, the intake of food, and the ratio of feed conversion were calculated. Results Cu-NP administration has improved the blood profile, lymphoid organs index weight in Cu-NP treated broilers in relation to control and CuSO4 treated groups. Conversely, erythrocyte sedimentation rate, H/L ratio, MDA, and corticosterone hormone were reduced by Cu-NP treatment. Moreover, Cu-NP has a positive effect on all behavioral patterns more than other groups which significantly reflect