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Schwannomatosis is a relatively rare disorder in which the main goal is to preserve neurological function.Bacillus velezensis EB14, isolated from a leaf of Populus × jackii, possesses antagonistic activity against Sphaerulina musiva, a fungal pathogen of Populus sp. that causes leaf spots and stem cankers on poplars, limiting the utility of hybrid poplars as plantation trees. We sequenced the genome of B. velezensis EB14 to gain insights into the underlying basis of its antagonistic activity. Here, we report the complete genome sequence