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The well-known tumor suppressor transcriptional factor has been proposed to be one of the central hubs of a functionally related and hierarchically arranged gene network coordinating pubertal timing. Our previous studies revealed that is involved in the metabolic control of puberty. The current study aimed to investigate the underlying signaling pathway, through which mediated the metabolic control of puberty. We engineered the expression of and/or in GT1-7 cells to investigate the interaction between and system, and their impact on GnR