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33% organic carbon of the hydrochar was released. The aromaticity of DSAs decreased with increasing Tp and tr. The molecular weights of the DSAs and DPMs decreased with increasing Tp and tr. This study uncovered hydrochar's molecular structure as well as the content and properties of its labile fractions. Results can be used to help design specific hydrochars for potential applications, based on the trend of the molecular change under the condition of the studied parameters optimization to produce hydrochar in this study.The Amazon bas